Open Access Mini Review Article ID: GJBBS-2-108

    Important Factors Influencing Protein Crystallization

    Mohnad Abdalla*, Wafa Ali Eltayb, Abdus Samad, TIM Dafaalla

    The solution of crystallization problem was introduced around twenty years ago, with the introduction of crystallization screening methods. Here reported some of the factors which affect protein crystallization, solubility, Concentration of precipitant, concentration of macromolecule, ionic strength, pH, temperature, and organism source of macromolecules, reducing or oxidizing environment,additives, ligands, presence of substrates, inhibitors, coenzymes, metal ions and rate of equilibration.The aim of this paper to give very helpful advice for crystallization.

    Keywords: Proteins; Crystallization; Isoelectric point; pH; Solubility; Stability

    Published on: Sep 30, 2016 Pages: 25-28

    Full Text PDF Full Text HTML DOI: 10.17352/gjbbs.000008
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