2026-05-24T21:44:44Z
https://www.peertechzpublications.org/oai-pmh
oai:www.peertechzpublications.org:10.17352/2455-3492.000020
2017-06-10
PTZ.IJNNN:VOL3
Synthesis, Characterization and Imaging of Fluorescine Isothiocyanate Conjugated Magnetite Nanoparticles in MCF 7 Breast Cancer Cell Lines
Mohammad E KhosroshahiMaral Asemani<p><span style="font-size:11.0pt;font-family:
"Calibri","sans-serif";mso-ascii-theme-font:minor-latin;mso-fareast-font-family:
Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-theme-font:minor-latin;
mso-bidi-font-family:Gautami;mso-bidi-theme-font:minor-bidi;mso-ansi-language:
EN-US;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-US">In this work we
describe fabrication, characterization and possible application of FITC (</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";
mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:
minor-latin;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:Gautami;
mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-GB;mso-fareast-language:
EN-US;mso-bidi-language:AR-SA" lang="EN-GB">fluorescine isothiocyanate</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";
mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:
minor-latin;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:Gautami;
mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language:
EN-US;mso-bidi-language:AR-SA" lang="EN-US">) conjugated magnetite nanoparticles (MNPs) for
biomedical applications such as imaging of cancer cells. The MNPs possessed
octahedral-like geometry with almost completely dispersed distribution with
high saturation magnetization. The final FMNPs (</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";mso-ascii-theme-font:
minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;
mso-hansi-theme-font:minor-latin;mso-bidi-font-family:Gautami;mso-bidi-theme-font:
minor-bidi;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:
AR-SA" lang="EN-GB">fluorescine isothiocyanate </span><span style="font-size:
11.0pt;font-family:"Calibri","sans-serif";mso-ascii-theme-font:minor-latin;
mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-theme-font:
minor-latin;mso-bidi-font-family:Gautami;mso-bidi-theme-font:minor-bidi;
mso-ansi-language:EN-US;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-US">magnetite
nanoparticles) absorption band showed 30 nm red shift towards longer wavelength
compared to bare MNP and the laser-induced fluorescence was observed at 518 nm.
The MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) results
showed that FITC conjugation diminishes the toxicity MNPs mainly due to the
reduction of surface charge. Fluorescence microscopy confirmed the uptake and
distribution of FMNPs in MCF 7 (Michigan Cancer Foundation-7) breast cancer
cells, which suggests that it can be utilized for applications such as a
magnetic fluorescent probe for bioassay.</span><br></p>
International Journal of Nanomaterials, Nanotechnology and Nanomedicine - Peertechz Publications
2017-06-10
Research Article
https://doi.org/10.17352/2455-3492.000020
en
Copyright © Mohammad E Khosroshahi et al.