2026-05-02T03:40:15Z https://www.peertechzpublications.org/oai-pmh

oai:www.peertechzpublications.org:10.17352/ab.000011 2025-09-20 PTZ.AB:VOL8

Development of a High-throughput Assay to Identify Novel Reactivators of Acetylcholinesterase Becky L Hood Stefan Vasile Gideon ShapiroJames D Talton<p>Acetylcholinesterase (AChE) is a key enzyme in the nervous system capable of breaking down acetylcholine (ACh). ACh is a critical neurotransmitter that signals between nerve cells and muscles. Organophosphates (OPs), used as insecticides and nerve agents, irreversibly inhibit AChE by forming a covalent bond with the enzyme’s active site. Since exposure to these nerve agents can quickly lead to death, many researchers have strived to sdiscover new drugs that will reverse the inhibition of AChE by such agents. The goal was to develop a high-throughput miniaturized assay for testing large numbers of compounds for AChE reactivation quickly and reliably. Here, we describe our miniaturized assay that utilises the sarin surrogate nitrophenyl isopropyl methylphosphonate (NIMP) in an Amplex Red coupled assay and its validation with 42 compounds selected from an in silico virtual screen.&nbsp;</p> Archive of Biochemistry - Peertechz Publications 2025-09-20 Research Article https://doi.org/10.17352/ab.000011 en Copyright © Becky L Hood et al.