2026-05-20T10:48:31Z https://www.peertechzpublications.org/oai-pmh

oai:www.peertechzpublications.org:10.17352/ams.000042 2024-03-07 PTZ.AMS:VOL8

Fluorescence of plankton fish eggs of Black Sea Mullus barbatus ponticus for test-analysis of the cell fertilization and development Victoria V Roshchina Valerii A Yashin Tatyana N PetrovaVladimir I Maltsev<p>The autofluorescence of fish eggs from the Black Sea Mullus barbatus ponticus Essipov was studied in order to identify the state of their fertilization and development in the ichthyoplankton. The study differed from the earlier genetic fish investigations with artificial fluorescent dyes. In our experiments, among the eggs were the objects with various stages of development: from unfertilized and just-fertilized cells emitted in blue to well-seen embryos that fluoresced in red. Red-fluorescing embryonal probes were peculiar to living eggs, unlike blue-fluorescing cells with damaged structures. The fluorescent method (luminescence microscopy with or without microspectrofluorimetry) allows us to identify the earlier stages of development of the fish eggs. The autofluorescence of the fish eggs of the species studied was possible to recommend for the testing of fertilized and living eggs at the early stages of development that may be applied to the fish industry in the future.</p> Annals of Marine Science - Peertechz Publications 2024-03-07 Research Article https://doi.org/10.17352/ams.000042 en Copyright © Victoria V Roshchina et al.