2026-06-09T19:53:25Z https://www.peertechzpublications.org/oai-pmh

oai:www.peertechzpublications.org:10.17352/sscrt.000008 2016-12-29 PTZ.SSCRT:VOL2

Adipose Derived Mesenchymal Stem Cell Differentiation into Adipogenic and Osteogenic Stem Cells Hassan IH El Sayyad1 Mohamed A Sobh2 Soad A Khalifa1Omnia KR El-Sayyad<p><strong>Objective: </strong>Lipoaspiration of human breast fats are important source of adipocyte stem cells (hAMSCs) which play a great role in regenerative medicine. The present study illustrates its capability of its transformation and characterization of adipocyte, osteogenic or chondrogenic cells.</p><p><strong>Methods and results: </strong>The hAMSCs were positive for CD13, CD29, CD105 and CD90 and negative CD34 and CD 14. The hAMSCs were cultured in adipogenic or osteogenic culture for 4,7,14 &amp; 21 days. Gene expression for adipogenic (PCR of leptin, peroxisome proliferator-activated receptor-γ and lipoprotein lipase) and osteogenic (osteocalcin) cells were carried out. Biochemical assessments of adipogenic (lipoprotein lipase enzyme and glycerol-3-phosphate dehydrogenase) and osteogenic (alkaline phosphatase, B-galactosidase and calcium content) markers. Also, light and transmission electron microscopic investigation of adipocyte stem cell culture were investigated at 4,7,14 &amp; 21 days in both two models. Adipocyte derived from hAMSCs displayed fi broblastic morphology and confl uency at 7 days and fl at-shape with positive oil red staining at 14 &amp;21 days. At ultrastructural level, the adipocyte derived from hAMSCs exhibited ideal structure. Also, it showed adipogenic gene expression and&nbsp; biochemical investigation. Similar was observed of its osteogenic affi nity of bone cells derived from hAMSCs.</p><p><strong>Conclusion: </strong>The authors concluded that adipocyte stem cells are capable of differentiating into adipocyte, osteoblast and chondroblast depending on the culture medium and are promising in regenerating medicine.</p> Studies on Stem Cells Research and Therapy - Peertechz Publications 2016-12-29 Research Article https://doi.org/10.17352/sscrt.000008 en Copyright © Hassan IH El Sayyad1 et al.