https://www.chemisgroup.us/journals/open-journal-of-analytical-and-bioanalytical-chemistry A Peertechz Open Access Journal en-us 10 Mar, 2026 https://www.chemisgroup.us/articles/OJABC-10-134.php Two-dimensional liquid chromatography (2D-LC) represents a significant advancement in food analysis, addressing the limitations of one-dimensional liquid chromatography (1D-LC) in separating complex food matrices. Food analysis plays a critical role in assessing the components, physicochemical properties, sensory qualities, and contamination risks of food products. Given the rising interest in identifying bioactive compounds in food due to their health benefits, the need for enhanced analytical techniques has become more pressing. Traditional 1D-LC often struggles to resolve closely related and co-eluting species in complex food samples, necessitating methods with greater resolving power. 2D-LC offers a valuable alternative by coupling two independent separation processes, thereby significantly increasing separation efficiency. This approach is particularly effective when paired with mass spectrometry (MS), which enhances detection sensitivity and specificity, allowing for more accurate structural identification. Moreover, 2D-LC has proven instrumental in environmental analysis, particularly in detecting and characterizing organic micropollutants (OMPs) at trace levels. As the demand for comprehensive food analysis and environmental monitoring grows, 2D-LC coupled with MS is poised to play an increasingly vital role in ensuring food safety, quality, and informed consumer choices. Therefore, this review briefly introduces the working principle of 2D-LC concerning its application in the field of food and environmental analysis in recent years. 25 Mar, 2025 https://www.chemisgroup.us/articles/OJABC-9-133.php Saliva glucose detection using QCM technology has garnered significant attention of non-invasive blood glucose monitoring. Introducing antifouling materials is a pivotal strategy for addressing the issue of indiscriminate protein adsorption in saliva. Nonetheless, equipping surfaces with antifouling properties often comes at the cost of compromising the sensitivity of QCM tests. To tackle this challenge, we engineered an innovative antifouling hydrogel that integrates gold nanoparticles functionalized with L-cysteine (Au-Cys). When immobilized on quartz chips, this hydrogel not only enhanced glucose sensitivity but also significantly reduced nonspecific protein adsorption . The sensitivity of hydrogels without Au-Cys increased from 0.34 Hz/(mg/L) to 0.45 Hz/(mg/L) of hydrogels containing Au-Cys. Additionally, protein adhesion of bovine serum albumin, lysozyme, and mucin was markedly decreased by approximately 25, 10, and 50 times, respectively. 11 Sep, 2024 https://www.chemisgroup.us/articles/OJABC-8-132.php This study investigates the relationship between organizational behavior and academic excellence in an intercultural university in Peru. Using a qualitative approach, various aspects of organizational behavior, such as institutional culture, leadership, motivation, and teamwork, and their influence on academic performance in an intercultural context were examined. Data were collected through surveys and interviews with students, faculty, and administrative staff of the selected university. The results show that an inclusive organizational culture, participative leadership, intrinsic motivation, and effective teamwork are key factors for academic excellence in cross-cultural settings. These findings have important implications for university management and the design of policies and programs aimed at improving academic performance and promoting inclusion in intercultural contexts in Peru and beyond. 30 Apr, 2024 https://www.chemisgroup.us/articles/OJABC-8-131.php Fluoroquinolones (FQs) are widely used antibiotics for therapeutic purposes, yet their extensive usage and unwise disposal led to antibiotic resistance as well as their widespread across the environment and becoming a major contaminates in water bodies as the wastewater treatment plants are not capable removing of FQs completely. Knowing the latter, the researchers endeavored to find techniques for the detection and removal of such contaminates from the disposed water from different facilities. This review highlights some of the recently and currently developed techniques for detection and removal. It discusses also different adsorbents used to remove FQs from wastewater. All in all, the removal of such leading contaminates from water would inhibit their spread across different environmental spheres thus promoting better environmental health. 16 Mar, 2024 https://www.chemisgroup.us/articles/OJABC-8-130.php Ultra HPLC-based analytical method analytical method was developed for the analysis of pymetrozine residues in different rice matrices. Chromatography separation was carried out in XR-ODS II: 150mm X 2.0mm, 5µm column in the mobile phase of water and methanol (60:40), and the resultant chromatograms were detected in a Photodiode Array (PDA). The retention time for pymetrozine neat standard and also matrix match standards was arrived at 4.00 min ± 0.5 min. The pymetrozine at the concentrations of 0.01, 0.05, 0.10, 0.25, 0.50, 0.75, and 1.00 µg/ml and the calibration curve of y = 2E+07x + 19188 obtained with a goodness of fit (R²) 0.995. The samples were extracted using the AOAC QuEChERS method with slight modifications. The harvest time residues for the different rice matrices were below the tolerance limit of pymetrozine. The residues of pymetrozine at 7, 15, and 30 days after the rice harvest in paddy seed, single-polished, double-polished rice, bran, and dehulled rice were not detected. From the developed analytical method, the residues of pymetrozine can be detected in different rice matrices. 30 May, 2023 https://www.chemisgroup.us/articles/OJABC-7-129.php Exposure to radioactive pollutants such as Iodine-135 I135 seriously threatens public health and environmental balance. Monitoring and managing these pollutants require expensive economic equipment that is not suitable for low-income countries such as Egypt. Therefore, trying to derive a mathematical model that estimates the concentrations of these radioactive pollutants with high accuracy and a low relative error coefficient compared to the actually measured values ​​is very important. Therefore a mathematical Gaussian model was received to estimate the concentrations of I135 emitted from the research nuclear reactor in the Inshas region in Egypt using different shapes of plume rise in unstable conditions. A comparison between the derived model and its maximum values with observed concentrations data measuring on Egyptian Atomic Energy Authority for I135 in unstable conditions has been found. The derived model may be applied to estimate and predict the emissions of any radioactive pollutant for any similar area and similar type of used reactor, which provides high-precision technology with zero economic cost. The statistical technique shows that the entire Gaussian model and its maximum inside a factor of two with observed concentration data achieved 98%. In addition, the statistics show that all the Gaussian plume models and their maximum have a correlation coefficient of about 0.95%. Also, the normalized mean square error. And the fraction bias. are near-zero values in all Gaussian models and their maximum. 09 Feb, 2023 https://www.chemisgroup.us/articles/OJABC-7-128.php Improving the efficiency of the electrode materials is critical for achieving high performance in supercapacitors. Porous carbon with appropriate designs is dependable for better electrochemical capacitors. In this work, we improved Zea mays (maize) cobs as a potentially plentiful precursor for generating porous carbon supercapacitor applications. The physical and chemical properties of the synthesized materials were determined using several approaches, including structure, morphology, chemical composition, and electrochemical performance. The chemical analyses revealed an oxygen-based surface structure, while structural analysis revealed a BET-specific surface area of 1443.94 m3/g and a pore volume of 0.7915 cm3/g. Symmetric devices based on the materials generated had a specific capacitance of 358.7F/g, an energy density of 12.45 Wh/kg and a power density of 250 W/kg at 0.5A/g. The as-prepared electrodes demonstrated exceptional stability, with a capacitance retention of 99% at the maximum potential for a total of 130 hours of testing. 31 Dec, 2022 https://www.chemisgroup.us/articles/OJABC-6-127.php Diastase is an enzyme that is found naturally in honey and degrades over time, especially when exposed to heat. Diastase can be used to indicate the age and exposure of honey to heat. However, they play a very significant role in honey quality. This study aimed to assess the diastase levels of different floral honey in potential areas of the Oromia region. In this study diastase activity, free acidity, and pH contents of different floral kinds of honey of potential areas in the Oromia region of Ethiopia were investigated based on the IHC- 2009- method. The honey pollen spectra were also characterized. When compared to other flora honey collected from the study area, the diastase activity of Erica arborea honey revealed significantly different (p < 0.05). It ranged from 4.61 ± 1.50 to 12.75 ± 4.78 Schade unit. The results of the pH value and free acidity analyses test demonstrated that the honey samples used in this investigation were fresh which ranged from 3.22 ± 0.13 to 4.17 ± 0.97 and 22.43 ± 6.37 to 35.10 ± 10.51 meq/kg honey, respectively. Honey bees’ geographical race, location and harvesting time affected diastase activities. However, it was not possible to identify the real factor influencing its diastase activities which needs further study at different agroecologies for different floral kinds of honey of the country. 31 Oct, 2022 https://www.chemisgroup.us/articles/OJABC-6-126.php With the advancement in the field of agriculture and industrial regime, numerous metals such as lead, cadmium, mercury, zinc, copper, arsenic, etc. are released into the environment as effluent. These metal ions enter water bodies and generate many health issues. Considering their harmful impact on human lives, numerous fluorescent probes have been developed in recent years to detect the presence of these metal ions. The fluorescent probes are used owing to their ease of operation, good selectivity, and high sensitivity. The interaction between functional groups present in the structural framework of the probe and the empty orbitals of metal ions is responsible for the chemo selectivity of the probe. This report summarizes the organic probes which possess benzofuran and naphthofuran moieties and act as fluorescent chemosensors toward metal ions. The selectivity and sensitivity of these chemosensors along with the limit of detection have also been discussed. 27 Apr, 2022 https://www.chemisgroup.us/articles/OJABC-6-125.php Studies were conducted to evaluate insecticide residues of Chlorantraniliprole in pigeon pea succulent pods after foliar application. Chlorantraniliprole was sprayed at 0.6ml/l on pigeon pea crop at the pod formation stage to control pod borers like Helicoverpa armigera and Maruca vitrata. Samples were drawn at 0, 1, 5, 10, 15, 20, 25, and 30 days after spray. A validated liquid chromatography method with a Photodiode Array (PDA) detector was used for the residue analysis of chlorantraniliprole. Separation was achieved through the C18 column in the reverse phase. The calibration graphs of chlorantraniliprole in acetonitrile solvent or five blank matrices were linear within the tested interval of 0.1 to 1ppm with a coefficient of determination over 0.9990. Dissipation of chlorantraniliprole was studied in first-order kinetic models (for which the coefficient of determination, R2 was 0.8684). Residues of chlorantraniliprole was 3.57, 3.16, 1.61, 1.13, 0.49, 0.21, 0.05 and 0.00mg/kg at 0, 1, 5, 10, 15, 20, 25 and 30 days after spraying. The residue level reached below the tolerance limit (MRL) (2mg/kg BW) of Codex Alimentarius Commission (CAC) on Pesticide Residues after 5 days of spray and below the detectable level after 25 days of spray in succulent pods and recorded a biological half-life of 4.36 days. 07 Mar, 2022 https://www.chemisgroup.us/articles/OJABC-6-124.php The molecule of transketolase is a dimer with structurally and functionally identical subunits. Its active sites are located in the region of intersubunit contact, which has been shown also for other thiamine enzymes. This leads to the reciprocal influence of active sites in the binding of cofactors and during catalysis. In this review, it is shown that the functional non-equivalence of the active sites of transketolase from Saccharomyces cerevisiae is initially formed upon the binding of the first cofactor, a divalent cation, not thiamine diphosphate, as previously thought. An attempt was made to find the reason for the differences between catalytic measurements and crystallographic data on the possible part-of-the-sites reactivity of the functioning of transketolase. At the same time, the difference in the amplitudes of dichroic absorption during the binding of reversibly and irreversibly splitting substrates has nothing to do with the flip-flop mechanism. It was also shown that with an increase in the concentration of substrates, a sharp decrease in activity occurs, which is explained by a switch from the simultaneous binding of the substrate in two active sites to its alternate binding both in one-substrate and two-substrate reactions. This fact could also be the reason for the rejection of the flip-flop mechanism of catalysis by transketolase. The mechanism may be similar to human transketolase, which may have clinical application. 04 Feb, 2022 https://www.chemisgroup.us/articles/OJABC-6-123.php ZSM-5 `is a porous zeolite material that reveals good activity for the adsorption of heavy metals and other contaminants for effluent purification. In this scientific research, we synthesized the novel CaO-CdO/ZSM-5 zeolite nanocomposite adsorbent and studied the effects of different parameters on the adsorption of Pb2+ ions from water media for the first time. About 99.2% adsorption removal efficiency was obtained after a contact time of 60 min and an adsorbent amount of 0.1g/L for an initial Pb2+ concentration of 200mg/L at pH 8 and temperature of 60oC. The adsorption kinetic fit well via the pseudo-second-order model. Furthermore, the adsorption results were reproduced using the Langmuir isotherm with a maximum adsorption capacity of mg/g. The thermodynamic data displayed that the adsorption of Pb2+ ions over the CaO-CdO/ZSM-5 nanocomposite was endothermic and spontaneous. 06 Oct, 2020 https://www.chemisgroup.us/articles/OJABC-4-122.php Dissolution testing is an essential tool in the pharmaceutical industry and is used in formulation and process development, in monitoring of the manufacturing process, as a quality control test, to predict the in vivo performance. The purpose of this study is to evaluate the behaviour of the test and reference products using conventional dissolution apparatus basket and paddle, with tendency to evaluate the dependence of the in vitro dissolution of the dissolution test conditions (dissolution apparatus, medium, agitation, pH). Experimental design (ED) approach has been employed for assessment of the discriminatory properties of different dissolution conditions. The responses statistically evaluated were: f2 similarity factor and the difference in the dissolution rate between the test and reference product, expressed in percentage, at every time point. Furthermore, this study focusses on developing a statistically reliable mathematical model for predicting discriminatory experimental conditions. 17 Sep, 2020 https://www.chemisgroup.us/articles/OJABC-4-121.php Urinary creatinine is measured to assess kidney function and also as part of sample validity testing in drugs of abuse. Creatinine methods based on alkaline picrate Jaffé’s reaction require extra cleaning on chemistry analysers to minimise any interference from picric acid and sodium hydroxide on other reagents on board. Enzymatic methods reagents are not as invasive and more specific. Siemens enzymatic method is more sensitive and specific when compared to Thermo Fisher alkaline picrate method but there were no available analytical parameters to setup the Siemens enzymatic method on Beckman-Coulter AU5800 analyser as an open system analyser. Emulating setup parameters from Siemens chemistry analysers did not work. The analytical parameters were developed through systematic testing using different settings to adopt and optimise the method. A full correlation was performed using the developed parameters with alkaline picrate method. The Deming regression weighted analysis for 438 samples showed a good correlation at a 95% confidence interval. Slope is 1.029 to 1.041, Y-intercept when X=0.0 is -0.9156 to -0.7481 and correlation coefficient (r) is 0.998. Alkaline picrate method Mean and SD is 12.059 and 7.248 respectively and for the Enzymatic method is 11.653 and 7.504 respectively. Many interferences from drugs and other substances are eliminated when using the enzymatic method and the stability of other reagents on-board improved because the reagents used in the enzymatic method are less invasive. 18 Aug, 2020 https://www.chemisgroup.us/articles/OJABC-4-120.php It was previously shown that in baker’s yeast Saccharomyces cerevisiae, transketolase can exist not only free, but in complex with RNA. The complex does not possess transketolase activity [N.K. Tikhomirova, G.A. Kochetov, A new method of isolation and a new form of transketolase from baker’s yeast, Biokhimiia 56 (1991) 1123-1130]. We discovered that this RNA is a ribozyme which catalyzes the interconversion of glyceraldehyde 3-phosphate (G3P) and dihydroxyacetone 3-phosphate (DHAP), i.e. acting as triosephosphate isomerase (TPI). It also catalyzes an unusual reaction of ribose 5-phosphate (R5P) decomposition to G3P ​​and erythrose. TPI-ribozyme was found in baker’s yeast not only in complex with transketolase, but also in free form. Transketolase-RNA complex was easily isolated on an immunoaffinity column with antibodies to transketolase. TPI-ribozyme consists of 87 nucleotides and has a molecular weight of 26.6 kDa. The optimum of pH-activity is 7.5 for DHAP, 6.7 for R5P and 9.0 for G3P. Km and Vmax are accordingly 0.29 mM and 2.6 U/mg for DHAP, 22 mM and 0.65 U/mg for R5P, 0.05 mM and 4.3 U/mg at pH 7.6 and 0.11 mM and 16 U/mg at pH 9.0 for G3P. These kinetic characteristics are the same for free RNA and in the complex with transketolase. Ki for RNA binding to transketolase was 1.0 μM. Accordingly, the TPI-ribozyme performs a dual function – it shows TPI activity and blocks the work of transketolase, thereby switching the metabolic process to glycolysis. The location of the TPI-ribozyme gene is determined. Blocking the activity of transketolase by ribozyme may be of practical importance in medicine, particularly, in cancer therapy. 07 Aug, 2020 https://www.chemisgroup.us/articles/OJABC-4-119.php The concentration of five soil heavy metals ions (Cr6+, Cu2+, Cd2+, Pb2+, and Ni2+) was measured in eleven sampling sites along Old Panteka market Kaduna from two different depths. These chemical elements in the samples were determined using atomic absorption spectrometer. The assessment of heavy metal pollution was derived using the Enrichment Factors (EF) and geoaccumulation index (Igeo). This study revealed that the soil is predominantly polluted by Pb2+>Cu2+>Cd2+>Ni2+>Cr6+ and Cu2+>Pb2+>Cd2+>Ni2+>Cr6+ metal ions at 0-5 cm and 5-10 cm depths respectively. As recorded the highest EF value of 29.63 and 20.54 for Pb and Cu at 0-5 cm and 5-10 cm depths respectively followed by Cu (17.13), Cd (10.07), Ni (0.99) and Cr (0.53) at 0-5 cm and Pb (19.68), Cd (12.47), Ni (1.19) and Cr (0.55) at 5-10 cm depths respectively, and the mean Igeo provided the same trend of pollution levels as in the case of the EF, which indicates that the highest level goes to Pb (1.61) and Cu (1.58) at 0-5 cm; Cu (1.71) and Pb (1.50) at 5-10 cm depths respectively, which exhibits unpolluted to moderately polluted. Meanwhile, Ni recorded (0.15) and (0.22) at 0-5 cm and 5-10 cm depths respectively, while Cr recorded (-0.07) and (0.08) also at 0-5 cm and 5-10 cm depths respectively, which illustrates that both of these metals vary from unpolluted to moderately polluted. The concentrations of Cr, Cu, and Ni levels are below 0 at the control sites, which demonstrates background concentrations. Risk assessment results show high health risk to human adults and children due to metals’ exposure through contaminated soil ingestion. 29 Jun, 2020 https://www.chemisgroup.us/articles/OJABC-4-118.php Glutamic Acid Decarboxylase (GAD) is an enzyme that converts glutamate to γ-aminobutyric acid (GABA) both in the brain and pancreatic β-cells. Several analytical methods are described for quantitative assay of GAD, where little attention has been given to the enzyme regulation in tissues, in part, due to the complexity of the methods. In this study, a novel fluorimetric method based on changes of fluorescence intensities upon the addition of glutamate substrate into the assay mixture is described. Rat brain GAD was purified and the enzyme activity was determined fluorimetrically in different stages of the enzyme purification. Results showed that during purification steps, changes in fluorescence emission intensities (ΔF/min/mg protein) increased in the paralleled to purification folds of the enzyme. In support of these findings, the levels of CO2 production were measured by Warburg manometric method. The close correlation between the new fluorimetric method and the conventional manometric assay method was demonstrated. Because the proposed fluorimetric method is simple, accurate, and sensitive enough for measuring GAD activity in different stages of the enzyme purification, it would be recommended for the clinical and pharmaceutical investigations. 06 May, 2020 https://www.chemisgroup.us/articles/OJABC-4-117.php An HPLC method had been developed and validated for rapid simultaneous separation and determination of the two antihypertensive drugs, amlodipine and xipamide in human plasma within 5 minutes. 24 Dec, 2019 https://www.chemisgroup.us/articles/OJABC-3-116.php In scientific research, animal modelling of human disease is pivotal in both studying the mechanisms of the disease and developing potential therapies. An imbalance in the interaction between the endocannabinoid (eCB) and Neurotransmitter (NT) systems may play a role in the pathogenesis of neurological diseases, such as Parkinson’s Disease (PD). The major limitation of current neurochemical practice is that different assays are used to measure each class of NTs. 13 Dec, 2019 https://www.chemisgroup.us/articles/OJABC-3-115.php Extract of feverfew (Tanacetum parthenium) has anti-inflammatory effect with various therapeutic benefits. Alpha-unsaturated gamma-lactones including parthenolide were recognized as part of the major active ingredients but with undesirable allergic reactions. 06 Nov, 2019 https://www.chemisgroup.us/articles/OJABC-3-114.php A novel, rapid, precise and accurate stability indicating RP-HPLC method was developed and validated for the simultaneous estimation of Ibuprofen and Carisoprodol in combined pharmaceutical formulation. 05 Nov, 2019 https://www.chemisgroup.us/articles/OJABC-3-113.php A fast, sensitive and selective method has been developed for quantitative determination of residues of nineteen benzimidazoles in meat by speeding the productivity of the conventional liquid chromatographs. 17 Sep, 2019 https://www.chemisgroup.us/articles/OJABC-3-112.php Relevant: Is the study of the response of biological macromolecules to external stimuli. Often the reaction of macromolecules has an effect of structural self-regulation. In this case, their reaction is not only external infl uence, but also the spatial-temporal motions of the macromolecule. In this situation deserves the attention of electronic-conformational interactions of macromolecules. 05 Sep, 2019 https://www.chemisgroup.us/articles/OJABC-3-111.php Pregabalin is a structural analogue of, but functionally unrelated to, the naturally occurring neurotransmitter -aminobutyric acid (GABA) with potent analgesic, anticonvulsant and anxiolytic activity. The abuse potential of pregabalin is a well-documented with high risk of addiction that may be fatal with overdoses particularly, among opiate addicts and polydrug use dependency syndrome, consequently, it is very pertinent to determine pregabalin in a sensitive and selective manner. 08 Aug, 2019 https://www.chemisgroup.us/articles/OJABC-3-109.php A simple and reliable high-performance liquid chromatography with diode-array detection (HPLC-DAD) method was developed for simultaneous determination of 9 natural substances common in plants: three major catechins ((-) - epicatechin gallate, (-) - catechin, (-) - epigallocatechin), four major flavonoids (rutin, quercetin, myricetin, kaempferol), gallic and vanillic acid. The optimized method was carried out for 40 minutes with detection wavelengths of 278 and 368 nm, gradient elution system on a C18 reversed-phase column. The developed system was evaluated for several validation characteristics, as system suitability, specificity, linearity, limit of detections (LODs) and limit of quantifications (LOQs). The newly established HPLC method was proved to be specific, sensitive, linear and precise. The received results showed good chromatographic separation and assumed that the described method was validated. The HPLC-DAD method can be a useful tool for the quantitative and qualitative evaluation of the selected polyphenol compounds and can be improved in the future for the examination of the polyphenols of interests in individual herbal infusions. 07 Aug, 2019 https://www.chemisgroup.us/articles/OJABC-3-108.php Because of plants, health benefi ts consumption of herbal infusions as a dietary additive has increased in the last years. 16 Jul, 2019 https://www.peertechzpublications.org/articles/OJABC-3-107.pdf The new general criterion, distinguishing between non-redox and redox electrolytic systems, is based on the properties of the linear combination f12 = 2∙f(O) – f(H) of elemental balances: f1 = f(H) for Y1 = H, and f2 = f(O) for Y2 = O. The f12 is the primary form of the Generalized Electron Balance (f12 = prGEB), completing the set of balances needed to formulate a redox system, according to Generalized Approach to Electrolytic Systems (GATES). In the redox system, f12 is linearly independent on charge (f0 = ChB) and other elemental/core balances fk = f(Yk) (k=3,…,K), i.e., f0,f12,f3,…,fK form the set of independent balances needed to formulate the redox system. In a non-redox system, f12 is linearly dependent on the balances f0,f3,…,fK, i.e., f0,f3,…,fK form the set of K – 1 independent balances needed to formulation of the non-redox system. Further properties, resulting from the combination of these equations specified here, are involved with oxidation numbers (ONs) of elements in components forming a non-redox or redox system. In the GEB formulation, made according to Approach II to GEB, the prior knowledge of oxidation numbers for all elements of the system is not needed. Moreover, ‘oxidant’ and ‘reductant’ are derivative/redundant terms. The mathematical knowledge, required to answer the question put in the title, is comprised within the four basic, algebraic operations. These properties are illustrated on a complex (41 species) example of a dynamic D+T redox system, and its static non-redox (T, D) subsystems. 16 Jul, 2019 https://www.peertechzpublications.org/articles/OJABC-3-106.pdf Any electrolytic system in aqueous media is described with use of charge balance, f0 = ChB, and K elemental or core balances, fk = f(Yk) (k = 1,…,K). The balances: f1 = f(H) and f2 = f(O) are the basis to formulate the linear combination f12 = 2.f2 – f1 = 2.f(O) – f(H). For a redox system with K – K* electronactive elements (players), f12 is linearly independent on f0, f3,…, fK, i.e., the redox system is described by K independent balances f0, f12, f3,…, fK. 28 Jun, 2019 https://www.chemisgroup.us/articles/OJABC-3-105.php Pyridoxine (vitamin B6) plays an important role in the body’s metabolism. Reaction of azocoupling with diazoaryl compound underlies in a basis a known photometric method of its chemical analysis. This method is not selective. In the present work the test method of determination of the pyridoxine containing in a molecule hydroxyarylgroup, was proposed by them azocoupling with chromogenic indicator diazo test strip, consisting of a covalently fixed on cellulose reagent with functional diazoaryl group, attached to the cellulose carrier through 1,3-diphenoxypropane bridge. This test strip interaction with resulted in formation of cellulose tetradentate chelating agent, capable to enter the subsequent chromogenic reaction with copper (II) ion. Formation of blue-violet complex of azoderivative pyridoxine with copper was used for determination of the content of pyridoxine (vitamin B6) in a number of his pharmaceutical preparations. 11 Nov, 2017 https://www.chemisgroup.us/articles/OJABC-1-104.php Vitamins A (Retinol, retinal, and four carotenoids including beta carotene), B1 (Thiamine), B2 (Ribofl avin), B3 (Niacin, niacinamide, Nicotinamide riboside), B5 (Pantothenic acid), B6 (Pyridoxine, pyridoxamine, pyridoxal), B7 (Biotin), B9 (Folates), B12 (Cyanocobalamin, dhyroxocobalamin, 07 Oct, 2017 https://www.chemisgroup.us/articles/OJABC-1-103.php In the current image article, we present different computational and experimental vibrational biospectroscopy methods and techniques such as Fourier Transform–Near– Infrared (FT–NIR), Fourier Transform–Short–Wavelength Infrared (FT–SIR), Fourier Transform–Mid–Wavelength Infrared (FT–MIR), Fourier Transform–Long–Wavelength Infrared (FT–LIR), Fourier Transform–Far–Infrared (FT– FIR), Attenuated Total Refl ectance–Fourier Transform Infrared (ATR–FTIR) and Fourier Transform–Raman (FT– Raman) spectroscopies for human cancer cells in tumor tissues simulation, modeling, research, diagnosis and treatment (Figure 1) [1–90]. 22 Sep, 2017 https://www.chemisgroup.us/articles/OJABC-1-102.php Introduction: Rapid diagnosis is fundamental for epidemiological control of Cholera disease. Contradictorily the gold standard test (stool culture) takes several days. Finlay Vaccine Institute obtained an Immunoagglutination test for rapid diagnosis of Cholera (FCIT), based on a monoclonal antibody anti- LPS O1 coupled to latex particles. FCIT, includes a positive control (LPS O1 Ogawa), which quantifi cation is mandatory to obtain the registration of the test. 25 Aug, 2016 https://www.chemisgroup.us/articles/OJABC-1-101.php The number of trace metallic elements of biological importance is increasing and a wide range of elements need to be analyzed in blood, urine and tissues. There is often a natural limitation of sample volume particularly in the analysis of blood and tissue digest samples.